High Capacity cDNA Reverse Transcription Kit contains all components required for cDNA synthesis and can convert up to 2μg of total RNA into single-stranded cDNA in a single 20μL reaction. The reaction system can be expanded to 100μL, and 10μg cDNA can be obtained in a single reaction. Downstream experimental applications include real-time fluorescence quantitative PCR, standard PCR and gene chip research. This kit is ideal for obtaining cDNA.

• Linear target amplification in real-time fluorescence quantitative PCR
• Compared with other cDNA synthesis kits, it has higher yield, Accuracy and lower cost
• Dynamic range is 10 times larger than other kits
• Optimum reaction temperature: 37°C
• Final product size: long Up to 7kb

• Extensively validated against a variety of templates
• Using random primers, all types of RNA can be tested for the first time Strand cDNA synthesis. The kit has been extensively validated against a variety of RNA templates, including GC- and AU-rich targets, as well as low-level expressed RNAs. In addition, cRNA can also be efficiently generated through in vitro transcription of cDNA

PCR & real-time fluorescence quantitative PCR, reverse transcription