Thermo Scientific Pierce DAPI Nuclear Counterstain is used for fluorescent staining of fixed cells for DNA content and nuclei in cell imaging technology. Highly pure form of diamidino-2-phenylindole dye.

Available as a room temperature stable, ready-to-use solution: NucBlue Fixed Cell Ready Probes Reagent.
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DAPI (diamidino-2-phenylindole) is a blue fluorescent probe that, when When it selectively binds to the minor groove of double-stranded DNA, it emits bright fluorescence, which is approximately 20 times greater than the fluorescence in the unbound state. Its DNA selectivity and high cell permeability enable efficient staining of cell nuclei with minimal background from the cytoplasm. DAPI is the classic nuclear counterstain used in immunofluorescence microscopy and is an important component of high-content screening methods that require cellular quantification of DNA content.

Features of DAPI nuclear counterstain:

•DAPI dye—diamidino-2-phenylindole is a DNA-specific blue fluorescent Staining
• Convenience—Choose powder or easy-to-use DAPI dye solution (1 mg/mL)
• Cell Imaging—Cell-permeable dyes are effective Stain fixed cells and quantify DNA content
• Counterstain—ideal for parallel detection of specific targets with fluorescence microscopy or high content screening (HCS) fluorescent antibodies
< br>Characteristics of DAPI fluorescent dye:

•Other names:DAPI stain, DAPI dye, DNA content counterstain
•Chemical name:4,6'-Diamidino-2-phenylindole dihydrochloride; 4',6-Diamidino-2-phenylindole; 2-(4-amidophenyl)-6-indole Indoguanidine hydrochloride
•Molecular formula:C₁₆H₁₅N₅·2HCl
• Molecular weight:350.25
• Excitation wavelength:341±3 nm (nearly 360 nm when combined with dsDNA)
• Emission wavelength: b>452±3 nm (456 to 460 nm when bound to dsDNA)
• Extinction coefficient: at 347 nm> 30,600/M cm, dissolved in methanol
• < b>CAS Number:28718-90-3
• Purity: by HPLC, >95% at 240 nm (most lots >98%)< br>• Solubility:> 1 mg/mL, soluble in water; compound soluble in DMF, water and various non-phosphate aqueous buffers
• Reactive groups :None; binds to the minor groove of double-stranded DNA

As a counterstain for fluorescence imaging methods, DAPI is compatiblefluorescein and rhodamine dye-labeled antibodies and others Probes and Thermo Scientific DyLight Fluor. DAPI is more photostable than Hoechst dyes, but Hoechst 33342 can be used for live cell imaging, while DAPI is only suitable for fixed cells. DAPI is available as powdered solids and aqueous solutions.

Applications of DAPI stains:

• Detection of DNA in solution (Reference 4)
• Diagnosis of mycoplasma infections in cell cultures (Reference 5)
• In flow cytometry Measure nuclear content and classify chromosomes in cell analysis (Ref. 6)
• Assess apoptosis (Ref. 7)
• Detect nuclear and organoid DNA in immunofluorescence and in situ hybridization procedures (Ref. 2, 8 ; Reference 8)
• Reports also indicate that DAPI binds to polyphosphates and other polyanions (Reference 10), dextran sulfate (Reference 11), and SDS (Reference 12).

Related products
DAPI (4,'6-II Amidino-2-phenylindole dihydrochloride)