Thermo Scientific Restore Fluorescent Western Blotting Antibody Stripping Buffer is a gentle and highly effective reagent that quickly removes from western blots Primary antibodies and near-infrared (IR) dye-labeled secondary antibodies.

Restore Fluorescent Western Blot Stripping Buffer Features:

•Fast—stripping blots in just 15 minutes at room temperature
• Save Time—No need to run new gels and prepare new blots
• Save samples—Reprobe multiple targets on the same PVDF membrane
• Economical —Cost less than other commercially available stripping buffers
• Efficient—Effectively strips blots the first time

Restore Fluorescent Western Blot Stripping Buffer makes PVDF membranes Reusable, simplifying Western blot optimization and allowing the same blot to be reprobed with different primary antibodies to detect additional targets. Restore Fluorescent Western Blot Stripping Buffer is only suitable for use with low-fluorescence PVDF membranes (Cat. No. 22860).

Fluorescent Western Blotting is a powerful method for detecting multiple targets simultaneously. Restore Fluorescent Western Blot Stripping Buffer enables reprobing of PVDF membranes, saving time and cost. This is ideal when sample is limited and different primary antibodies need to be used for optimization or analysis.

Traditional stripping methods may alter or remove sample proteins on the PVDF membrane in an unfavorable way during the stripping process, or may only effectively remove low-affinity antibodies. In contrast, Restore Fluorescent Western Blot Stripping Buffer strips more than 90% of the time while reprobing efficiencies that rival or exceed those of other suppliers' formulations. Additionally, Restore Fluorescent Western Blot Stripping Buffer is conveniently stored at room temperature and is easy to use. Simply dilute the buffer 1:5 in water and incubate the membrane at room temperature for 5 to 20 minutes.