Thermo Scientific Pierce Sulfo-NHS is a chemical modification reagent used to convert carboxyl groups to amino-reactive NHS esters. For operations such as bioconjugation, cross-linking, labeling and fixation.

Features of Sulfo-NHS:

• Sulfo-NHS improves EDC-mediated coupling efficiency
• Ability to utilize any carboxyl-containing molecule to form amino-reactive NHS esters or Sulfo-NHS Esters
• Sulfo-NHS derivatives are usually directly soluble in water (can be added directly to physiological buffers) and cannot penetrate cell membranes (can be used to label cell surfaces)
• High-purity crystalline Sulfo-NHS can be used to generate High-quality activated derivatives

Sulfo-NHS (N-hydroxysuccinimide) can control and modify carbodiimide cross-linking reactions, including activated carboxylate (—COOH) To couple primary amine group (—NH2). Related derivatives can be conveniently synthesized by mixing Sulfo-NHS with a carboxyl-containing molecule and a dehydrating reagent such as carbodiimide EDC (EDAC). This method is the basis for preparing many types of protein labeling reagents, including amino-reactive fluorescent dyes, biotin affinity tags, and PEGylated compounds.

Applications:
• Improve EDC coupling reaction efficiency
• Convert carboxyl groups into amino-reactive Sulfo-NHS ester
• Coat proteins with carboxyl groups Efficient cross-linking between microbeads or surfaces
• Activation of nanoparticles using amino-reactive Sulfo-NHS ester

Specifications of Sulfo-NHS:
We follow the highest Standard production ofN-hydroxysuccinimide results in the most specific bioconjugates, ensuring data integrity and providing you with the highest level of experimental consistency. Each batch of Sulfo-NHS is tested and meets the following standards:

• Purity— Quantitative NMR detection purity ≥95% (the highest standard for cross-linking agent purity)
Average purity per batch is greater than 99%
• Solubility— Dissolves in deionized water at 2 mg/mL to form a clear, colorless solution
• Identification Testing —IR scan only shows the characteristic peak of N-hydroxysuccinimide

Product reference:
Cross-linking agent application guide— Search the latest references for this product

Related resources:

Cross-linking Overview< br>Cross-linking chemical principles

Related products:
EDC (carbodiimide cross-linking agent)
Bioconjugation technology (book)
Crosslinking agent list (selection guide)
NHS activated biotinylation reagent
NHS activated fluorescent labeling reagent
NHS activated agarose resin
NHS activated magnetic beads