Fixable Viability Dye eFluor™ 780 is a reactive dye that can be used before cryopreservation, fixation and/or permeabilization procedures Irreversibly label dead cells. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dye can be washed, fixed, permeabilized, and stained for intracellular antigens without reducing the staining intensity of dead cells. Therefore, when studying intracellular targets, use the Fixable Viability Dye to exclude dead cells from the analysis. Fixable Viability Dye can be used to label cells of all species.

Fixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line, with an emission peak of 780 nm, and can use a 780/60 bandpass filter (equivalent to APC-eFluor™ 780 or APC -Alexa Fluor™ 750) detection. Please make sure your instrument can detect the dye. For compensation, it is recommended to use a sample of cells of interest stained with Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, it is recommended to remove a small aliquot of cells and heat at 65°C for 1 minute, followed immediately by placing on ice for 1 minute. After the above treatment, the heat-inactivated cells can be mixed with viable cells at a ratio of 1:1 and stained using Fixable Viability Dye. Our detection shows that the compensation from most detectors is negligible, with the compensation from PE-Cyanine7 being higher, below 5%. Actual compensation values will depend on each researcher's specific instrument, filter set, and PMT voltage settings.

Fixable Viability Dye eFluor™ 780 is supplied as a pre-diluted solution prepared in high quality anhydrous DMSO. Store away from light and moisture. Store at -70°C using desiccant. Can be frozen and thawed up to 20 times. Allow vials to equilibrate to room temperature before opening.

Reported Applications
Flow Cytometry