Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotin used to label azide-containing molecules reagents, enabling biotin-based molecular detection and affinity purification via the Staudinger ligation strategy.

EZ-Link Phosphine-PEG3 Biotin Characteristics:

•Soluble—Easily soluble in water-soluble solvents (e.g., DMSO) for subsequent processing in Dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Compatible—Efficient chemical reactions under simple buffer conditions; no auxiliary reagents such as copper or reducing agents are required< br>• Chemistry—Phosphine reactive groups are specific for biological samples and are suitable for bioorthogonal azide labeling molecules, ensuring specific biotinylation
• PEG Spacers—Polyethylene glycol spacers help maintain the solubility of the labeled molecule and reduce the risk of affinity binding to avidin, strepavidin, or neutravidin. Steric hindrance.

When used in conjunction with an azide labeling strategy, this compound enables directional biotin labeling to aid in protein interactions and translation using streptavidin probes or streptavidin agarose resin. Post-modification for detection or affinity purification. By Staudinger reaction mechanism, the phosphine group of phosphine-PEG3-biotin is coupled to the azide group. Azide groups can be introduced into proteins or other cellular targets throughin vivo labeling using azide-tagged derivatives of naturally occurring metabolic building blocks. Neither phosphines nor azides are present in biological systems, so they contain chemoselective (mutually specific) linking pairs for labeling and conjugation.