Minimum Essential Medium (MEM) is a commonly used cell culture medium. MEM can be used to culture a variety of suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes wait. Gibco™ MEM Modified Medium is available in a variety of components for a wide range of cell culture applications. Use
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The improved form of this MEM is as follows:
Includes | Does not contain td> |
L-Glutamine | Gentamicin |
Proline | HEPES: |
Sodium pyruvate | Insulin |
Zinc | < /td> |
Provide complete< a href="http://www.invitrogen.cn/site.gateway.html?type=mediaForm&productId=246">Formula.
Gibco™ Richter's MEM is an improved formula of Eagle's MEM, which contains iron, zinc, putrescine, fatty acids and gentamicin. Richter's modification also contains extremely high concentrations of choline chloride and inositol. This product has been further modified to contain L-glutamine and does not contain gentamicin sulfate.
Product UsageProducts are for research use only. Not for use in diagnostic procedures.
Dual on-site cGMP manufacturingGibco™ Richter's MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ MEM Medium does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10%
Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.