Applied Biosystems™ TaqMan™ hPSC Scorecard™ Assay 384w validates and determines the pluripotency of human ES and iPS cell lines Pedigree bias. The 384-well plate contains four sets of 94 predetermined TaqMan™ gene expression assays (including endogenous controls) dried in the wells. The master mix is not supplied with this assay plate. For a kit that provides a test plate with a master mix, see TaqMan™ hPSC Scorecard™ Kit 384w. This product is also available in 2 x 96w fast spec.

• Evaluate the gene signature of four samples in one simple experiment
• Compare results to reference standards in the included hPSC Scorecard™ analysis software
• Determine those with trilineage differentiation Potential Cell Lines
• Evaluate induced differentiation specific to one germ layer
• Access validated content to increase confidence in your results

Assess the entire gene signature in a single experiment
TaqMan™ hPSC Scorecard™ Assay Plates help save time by providing pre-made assays in a stable, convenient dry format. Simply add TaqMan™ Master Mix to your cDNA of interest and transfer it to a 384-well plate using a multichannel pipette.

Compare results to reference standards
TaqMan™ hPSC Scorecard™ panels include access to proprietary cloud-based analysis software that allows you to view and export graphs of your results and forms, and generate reports. The software is compatible with seven Applied Biosystems™ qRT-PCR systems at no additional charge.

Identification of cell lines with tri-lineage differentiation potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your Analyze and determine whether your cell line is biased towards one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluation of induced differentiation specific to one germ layer
The panel includes more than 20 markers for each of the three germ layers that are cultured in monolayers have shown the expected response to directional differentiation. Use this assay panel to quickly evaluate time course data, culture conditions, and media formulations for target germ layers.

Access Validated Content
Panel content is based on published work (Bock et al., Cell 144, 439–452, 2011) and controls multiple human ES and iPS cell lines for validation.

Product Packaging Specifications
TaqMan™ hPSC Scorecard™ Assay Plate 384w includes:

•One 384-well plate with a total of 94 TaqMan™ gene expression assays ( 4 genomes/plate)
•One optical plate cover