Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit 2 x 96w FAST confirms pluripotency of ES and iPS cell lines and Identifying lineage bias. Two 96-well plates each contain 94 wells of dry predetermined TaqMan™ gene expression detection reagents (including endogenous reference). For your convenience, a premix is included. To purchase the assay panel separately, see TaqMan™ hPSC Scorecard™ Assay Panel 2 x 96w FAST. This product is also available in 384-well format.

• Evaluate the entire gene signature in one simple experiment
• Compare results to reference standards in the included hPSC Scorecard™ Analysis Software
• Identify cells with tri-lineage differentiation potential Lines
• Evaluate induced differentiation specific to one germ layer
• Access validated content to increase confidence in your results

Evaluate the entire gene signature in a single experiment
The TaqMan™ hPSC Scorecard™ Assay Panel provides pre-plated assays in a stable, convenient dry format to help save time. Simply add TaqMan™ Master Mix to your cDNA of interest and transfer it to a 96-well plate using a multichannel pipette.

Compare results to reference standards
The TaqMan™ hPSC Scorecard™ Assay Bundle includes access to proprietary cloud-based analysis software that allows you to view and export graphs of your results and forms, and generate reports. The software is compatible with seven Applied Biosystems™ qRT-PCR systems at no additional charge.

Identification of cell lines with tri-lineage differentiation potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your Analyze and determine whether your cell line is biased towards one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Assessing induced differentiation specific to one germ layer
The assay panel includes more than 20 markers for each of the three germ layers, which have been shown to The response to directed differentiation in monolayer cultures was as expected. Use this assay panel to rapidly evaluate time course data, culture conditions, and media formulations for germ layers of interest.

Access Validated Content
Assay panel content is based on published work (Bock et al., Cell 144, 439–452, 2011) and compared to multiple human ES and iPS Cell lines were validated.

Product Packaging Specifications
TaqMan™ hPSC Scorecard™ Assay Kit 2 x 96w FAST Includes:

•Two 94 TaqMan™ Gene Expression Assay Panels 96-well plate
• Two optical plate covers
• Two 1 mL vials TaqMan™ Fast Advanced Master Mix