Thermo Scientific EZ-Link NHS-PEG12-Biotin targets antibodies, proteins and any other macromolecules containing primary amines Simple and efficient biotin labeling. A hydrophilic polyethylene glycol (PEG) spacer arm transfers water solubility to the biotinylated molecule. For example, antibodies labeled with NHS-PEG4-biotin showed less aggregation when stored in solution than antibodies labeled with reagents with only hydrocarbon spacers. Specific labeling of cell surface proteins is another useful application for these water-soluble, membrane-impermeable reagents.

Thermo Scientific No-Weigh products are specialty reagents available in pre-portioned formats. Pre-weighed packaging eliminates repeated opening and closing of vials, preventing loss of reagent reactivity and contamination over time. With this format, a fresh vial of reagent can be used at a time, eliminating the hassle of weighing small amounts of reagent and reducing concerns about reagent stability.

EZ-Link NHS-PEG12-Biotin Features:

•Protein Labeling — Use streptavidin probe or resin to label antibodies or other Biotinylation of proteins for detection or purification
•Amine reactivity — Reacts with primary amines (-NH2), such as the side chain of lysine (K) or the amino terminus of a peptide
•PEGylated — The spacer arm contains a hydrophilic 12-unit polyethylene glycol (PEG) group
•Enhanced solubility — Polyethylene Diolation makes the biotinylated molecule water-soluble, helping to prevent aggregation of biotinylated antibodies stored in solution
•Irreversible — permanent amide bonds are formed; the spacer arm cannot be cleaved
•Long Range — The spacer arm (total length added to the target) is 56 Angstroms; this reduces steric hindrance to binding to the avidin molecule

NHS-PEG12 -Biotin is a long (56.0Å) pegylated, water-soluble, NHS ester biotinylation reagent used to label amines and maximize the solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and the N-terminal amino group at a pH value between 7 and 9 to form a stable amide bond. HydrophilicityPolyethylene glycol (PEG) The spacer arm imparts water solubility, transferring it to the biotinylated molecule, thereby reducing storage in solution aggregation of labeled proteins. The PEG spacer arm also provides a long, flexible linkage to the reagent to minimize steric hindrance when binding to the avidin molecule.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

N-hydroxysulfosuccinimide (NHS) ester of biotin is a biotinylation reagent Widely used type. NHS-activated biotin reacts efficiently with primary amine groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (such as antibodies) often have several primary amines that can be targeted for labeling, including the side chain of a lysine (K) residue and the N-terminus of each polypeptide.

BiotinThe various NHS ester reagents vary in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-Biotin is cell permeable but must be dissolved in organic solvents such as DMSO or DMF. Sulfo-NHS biotin (and biotin with a PEGylated spacer) can be dissolved directly in water but is not membrane permeable. Various types containing disulfide bonds can be cleaved using reducing agents, thereby separating the biotin groups from the labeled protein.