Thermo Scientific Pierce DSP (Lomant Reagent) is a water-insoluble, homobifunctional N-hydroxysuccinimide ester (NHS ester) ) cross-linker with thiol cleavability, primary amine reactivity, and is suitable for a variety of applications. DSP has an amine-reactive NHS ester at each end of the 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and release N-hydroxysuccinimide. Proteins, including antibodies, often have several primary amines in the side chains of lysine (K) residues and at the N-terminus of each polypeptide, and these primary amines can serve as targets for NHS ester cross-linking reagents. DSP is non-sulfonated and does not dissolve in water, so it must first be dissolved in an organic solvent before being added to the aqueous reaction mixture. Since DSP does not have charged groups, it is lipophilic and membrane permeable, and therefore can be used for intracellular and intramembrane coupling. The sulfonated analog of DSP (DTTSP) is water soluble. If the application requires a stable spacer arm that cannot be cleaved under reducing agent conditions, the non-cleavable analogue of the DSP cross-linker, DSS, can also be used.

Thermo Scientific No-Weigh products are professional reagents provided in pre-packaged formats. Pre-weighed packaging eliminates repeated opening and closing of vials, preventing loss of reagent reactivity and contamination over time. With this format, a fresh bottle of reagent can be used at a time, eliminating the hassle of weighing small amounts of reagent and reducing concerns about reagent stability.

DSP has an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds with the release of theN-hydroxysuccinimide leaving group. Proteins, including antibodies, often have several primary amines in the side chains of lysine (K) residues and at the N-terminus of each polypeptide, and these primary amines can serve as targets for NHS ester cross-linking reagents. If the application requires a stable spacer arm that cannot be cleaved under reducing agent conditions, the non-cleavable analogue of the DSP cross-linker, DSS, can also be used.

Characteristics of Lomant reagent:

•Reactive group:NHS ester (two terminal)
• Can react with the following groups: Amino group (primary amine)
• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
• pH When the value is 8.5, the disulfide bond in the spacer arm is easily cleaved by 10-50 mM DTT or TCEP
• The spacer arm can also be cleaved by reducing SDS-PAGE loading buffer
• Cleavable cross-linkers can Isolate cross-linked products
• Insoluble in water (dissolve in DMF or DMSO first); with DTSSP (Sulfo-DSP ) Comparable
• Permeable membrane for intracellular cross-linking

Applications:
• Intracellular before cell lysis and immunoprecipitation Chemical cross-linking of proteins
• “Fix” protein interactions to allow identification of weak or transient protein interactions
• Fix tissue for immunostaining without damaging RNA
• Perform protein cross-linking to allow identification of weak or transient protein interactions One-step reaction produces bioconjugates
• Immobilization of proteins to amine-coated surfaces

Properties of DSP
• Other names: Lomant reagent, DTSP
• Molecular formula: C₁₄H₁₆N₂O₈S₂< br>• Molecular weight: 404.42
• Spacer length: 12.0Å (8 atoms)
• Catalog number: 57757-57-0
• Reactive group: NHS ester at pH 7.0–9.0 Reacts with primary amines

DSP Specifications:
We manufacture DSP to higher specifications to produce highly specific bioconjugates, ensuring the integrity of your data and providing You provide a higher degree of consistency. Every batch of DSP is tested and meets the following minimum specifications.
•Identification:IR scan shows only peaks with structural and functional group characteristics of DSP
•Purity:> 95% (quantitative NMR method) (cross (Higher standards for cross-linking agent purity)

Product References
Cross-linking Agent Application Guide—Search for recent references for this product