Invitrogen TrueCut Cas9 protein v2, as a new generation of CRISPR Cas9 engineered protein, can bring maximum efficiency to gene editing. Its properties include:

• Consistently high editing efficiencies in all cell lines tested (including standard, immune, primary and stem cells), and higher editing efficiencies than the competition in difficult target genes 2x more product
• High quality—produced to strict ISO 13485 quality standards
• Provides solutions validated for a wide range of cell types to help you achieve success faster

TrueCut Cas9 protein v2 is a recombinant Streptococcus pyogenes Cas9 (wild-type) protein purified from Escherichia coli and can be used for genome editing based on CRISPR technology. The Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with guide RNA (gRNA) as a component of the CRISPR/Cas9 system. The introduction of nuclear localization signal (NLS) can help it enter the nucleus and increase the probability of genomic DNA cleavage.

•Direct transfection using liposome-mediated transfection reagent or electroporation
•Eliminate time-consuming cloning steps
•Available in 2 concentrations:
--1 μg/Lμ (for standard editing situations)
--5 μg/Lμ (for optimizing editing conditions in more difficult situations, such as in primary or embryonic cells, microinjection, or screening of multiple gRNAs at once When Sequencing
--For custom sizes or concentrations, please consult your salesperson

For options for CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready
a href="http://www.thermofisher.cn/us/en/home/life-science/genome-editing/geneart-crispr/crispr-libraries/trueguide-grnas.html">TrueGuide synthetic gRNA
2. Generate transfection-ready gRNA in just four hours, including with our GeneArt precision gRNA Template assembly completed by synthesis kit
3. Let our custom service team design, synthesize and purify in vitro (IVT) gRNA sequences for you