Thermo Scientific Pierce DSS (also known as disuccinimidyl suberate) is a non-cleavable membrane-penetrable Sexual cross-linker with an amine-reactive N-hydroxysuccinimide (NHS) ester at each terminus of the 8-carbon spacer arm.

NHS esters react with primary amines at pH 7-9 to form stable amide bonds and simultaneously release the N-hydroxysuccinimide leaving group. Proteins, including antibodies, often have several primary amines in the side chains of lysine (K) residues and at the N-terminus of each polypeptide, and these primary amines can serve as targets for NHS ester cross-linking reagents. DSS is first dissolved in an organic solvent (such as DMF or DMSO) and then added to the aqueous cross-linking reaction. The water-soluble analogue of DSS, BS3, is also suitable for applications requiring hydrophilic cross-linkers (e.g. to produce cell surface cross-links). The cross-linking activity of DSS and BS3 towards primary amines is essentially the same.

Characteristics of disuccinimide:

•Reactive group:NHS Ester(Both ends)
• Can react with the following groups: Amino groups (primary amines)
• Amine-reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule Reaction
• Membrane permeable, allows for intracellular cross-linking
• High-purity crystallization reagents can be used to generate high-purity conjugates
• Non-cleavable
• Water-insoluble (first dissolve in DMF or DMSO); compare with BS3 (Sulfo-DSS)

Applications:
• Chemical cross-linking of intracellular proteins prior to cell lysis and immunoprecipitation
• “Fixed” protein interactions to allow identification of weak or transient protein interactions
• Protein Cross-linking generates bioconjugates in a single-step reaction
• Immobilization of proteins to amine-coated surfaces

Properties of DSS
• Other names: Disamber Imide suberate
• Molecular formula: C₁₆H₂₀N₂O₈< br>• Molecular weight: 368.35
• Spacer length: Å (8 atoms)
• CAS number: 68528-80-3
• Reactive group: NHS ester, at pH 7.0–9.0 conditions Reacts with primary amines

DSS Specifications
We manufacture DSS to higher specifications to produce highly specific bioconjugates, ensuring the integrity of your data and providing You provide a higher degree of consistency. Every batch of DSS cross-linker is tested and meets the following minimum specifications:
•Identification: IR scan shows only peaks with structural and functional group characteristics of DSS
•Purity:> 90% (quantified by NMR method, which is a higher standard for cross-linking purity)
• Solubility:> 9.2 mg/mL, soluble in DMF and DMSO, clear colorless solution

Product References
Crosslinker Application Guide - Search the latest references for this product

Related Products< /b>
BS3 (bis(sulfosuccinimide)suberate)