Thermo Fisher A50575 25 μg TrueCut HiFi Cas9protein (1 μg/μL)
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A50575 25 μg TrueCut HiFi Cas9 protein (1 μg/μL)

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Invitrogen TrueCut HiFi Cas9 protein is a high-fidelity CRISPR Cas9 protein variant engineered to exhibit superior off-target characteristics while maintaining extremely high targeted editing efficiency. TrueCut HiFi Cas9 protein is ideal for applications sensitive to off-target events and when more precise editing is required.

Features of TrueCut HiFi Cas9 protein include:

  • Virtually no off-target events in all cell lines tested (including standard, immune, primary and stem cells)
  • Maintains extremely high targeted editing efficiency compared to TrueCut Cas9 protein v2
  • High quality—produced in accordance with strict ISO 13485 quality standards
  • Ready for transfection type, using electroporation or lipid-mediated transfection reagents
  • No time-consuming cloning steps required
  • Available in 2 concentrations:
    • 1 μg /μL, for standard editing situations
    • 5 μg/μL, for optimizing editing conditions in more difficult situations, such as in primary or embryonic cells, when microinjecting, or when screening multiple gRNA sequences at once See other concentrations>
    • For custom sizes or concentrations, please consult relevant Salesperson

TrueCut HiFi Cas9 protein is an engineered recombinant Streptococcus pyogenes Cas9 protein purified from E. coli and can be used for CRISPR-based Technologies for genome editing. The Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with guide RNA (gRNA) as a component of the CRISPR/Cas9 system. The introduction of nuclear localization signal (NLS) can help it enter the nucleus and increase the probability of genomic DNA cleavage.

Optional products for ordering CRISPR gRNA for use with TrueCut HiFi Cas9 protein:

  1. Order pre-designed TrueGuide for efficient knockout in humans or mice Synthesize gRNA
  2. Submit your own design for CRISPR sgRNA custom synthesis
  3. Use our free TrueDesign CRISPR Genome Editor software to easily complete your design and order custom gRNA to achieve Precision Genome Editing
  4. Generate transfection-ready gRNA in just four hours, including template assembly with our GeneArt Precision gRNA Synthesis Kit.