Pierce High Capacity EDTA Compatible Ni-IMAC Resin is designed for the purification of overexpressed His-tagged proteins from cell culture media. The matrix is a 6% cross-linked agarose that provides excellent performance for batch processing, gravity columns, and research/pilot scale FPLC column purification. The resin has high binding capacity (up to 80 mg protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metals and stabilize sensitive intracellular proteins.

Features of high-capacity EDTA compatible Ni-IMAC resin include:
• Chelating agent stabilization—use up to 20 mM EDTA and DTT without loss of performance
• Compatible with cell culture media such as Expi293 Compatible with purification of overexpressed secreted proteins in , ExpiCHO, and ExpiSf expression systems
• High protein binding capacity—His-tagged recombinant protein binding capacity >80 mg/mL
• Reusable—renewable using CIP protocols Up to 10 times

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping metal. Due to its chelating agent stability (EDTA tolerance), Pierce High Capacity EDTA Compatible Ni-IMAC Resin is particularly suitable for the purification of His-tagged proteins that are secreted in cell culture media or for the purification of intracellular proteins that require the presence of EDTA for stability and functionality. His-tagged proteins in cells. In both cases, large amounts of sample can be purified without stripping the nickel metal from the support, providing consistently high capabilities and yields.