Thermo Fisher A52699 1 x 1 mL TaqPath BactoPureMicrobiological testing master mix
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A52699 1 x 1 mLTaqPath BactoPure Microbial Test Master Mix

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TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid detection of low-level microorganisms, even in the presence of PCR inhibitors detection. All batches are functionally tested to help ensure Ct reproducibility and dynamic range consistency across multiple assay batches. This generic reagent is labeled "For Laboratory Use" and is manufactured in an ISO 13485-certified FDA-registered facility that complies with current Good Manufacturing Practice (cGMP) principles.

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Benefits of TaqPath BactoPure Microbial Test Master Mix
• Tests for bacterial, fungal, mammalian, viral DNA and antibiotic resistance markers Consistent detection at low levels
• Tolerant to PCR inhibitors commonly found in purified samples and crude lysates in biopharmaceutical, molecular diagnostics and research applications
Wide linear dynamic range* (up to 8 log levels) enables accurate detection of low and high concentration DNA samples
For multiple pathways Assays optimized—available in two formulations to detect 1 to 4 targets per reaction
Compliance Pack contains the most commonly requested documents and is available below

Two formulations
The master mix is available in two formulations:
• TaqPath Bactopure Microbial Detection Master Mix containing ROX dye as an inert reference dye is recommended for use in single Pass (1 probe) and up to three pass (3 probes) reactions
• TaqPath Bactopure Microbial Detection Master Mix (ROX-free), does not contain inert reference dye, recommended for use with JUN (or similar emission wavelength) dyes, or for advanced multi-pass detection (>3 targets)

Consistent low-level detection
TaqPath BactoPure Microbial Detection Master Mix Optimized , which enables highly reproducible, low-level detection from a variety of samples. While other commercially available mixes contain DNA background that can lead to ambiguous results (below), each batch of TaqPath BactoPure Master Mix is tested in a panel of reagents to demonstrate that it is free of this background prior to release. This allows TaqPath BactoPure Master Mix to reliably detect and quantify low-concentration samples, as shown below. The graph below also shows consistent Cq results from multiple assays of three unique batches, maximizing the confidence of your results and highlighting the excellent batch-to-batch consistency of TaqPath BactoPure Master Mix.

Tolerant to PCR Inhibitors
The unique and proprietary TaqPath BactoPure Master Mix formulation provides robust performance even in the presence of substances that inhibit PCR (below), thus Efficient detection of templates in purified DNA and crude lysates, as shown below.

Wide Linear Dynamic Range
TaqPath BactoPure Microbial Detection Master Mix has been validated to have a wide linear dynamic range (up to 8 log units), thus Capable of accurate detection using low and high DNA concentration samples. This reagent can be used on a variety of qPCR platforms and can be used under fast or standard cycling conditions. The graph below shows the excellent PCR linearity of the whole bacteria assay over an input range of 8 log units.

Optimized for multi-pass detection
TaqPath BactoPure Microbial Assay Master Mix is validated for multi-pass detection of up to 4 targets in a single reaction, enabling simultaneous Run additional assays and/or control assays to increase efficiency. The figure below illustrates this multipass assay capability by comparing performance between single pass and multipass reactions.

General Purpose Reagents Labeled 'For Laboratory Use' and manufactured in accordance with cGMP
TaqPath Master Mixes are general purpose reagents, labeled 'For Laboratory Use' and manufactured under ISO Manufactured in a 13485 certified and FDA registered facility that complies with cGMP principles.

Compliance Pack
To effectively support your compliance needs, the most commonly requested documents are provided below.
• Related to Current Good Manufacturing Practice (cGMP) compliance for TaqPath Master Mix Quality Statement
• Detailed batch-specific Certificate of Inspection (COA) (see "Documents & Downloads" below ” section)
Product Stability Statement
• Supply Supplier evaluation support
CompletedSelf-Assessment
ISO Certificate
• For inquiries regarding EU REACH compliance, please email LSGchemicalCompliance@thermofisher.com< br> • BSE/TSE Statement
Change Control and Notification Registration
Don't find the compliance document you need? Technical support team will be at your disposal at any time help.

Learn more about multipath assays, TaqPath probes, and how our products can help your project ›

&ast; Dynamic range is a measure of the concentration of template in the method and sample, as well as the master mix function of the recipe; therefore each result may vary.

For Laboratory Use