MEGAclear™ kits are designed for rapid purification of any large-scale transcription reaction. MEGAclear™ kits effectively isolate RNA from unincorporated NTP, enzyme and buffer components. MEGAclear™ Kit Benefits:

• Purify transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes and buffer components
• Inputs from 1 ng to 500 µg Medium-High Recovery
• Suitable for purified aRNA amplification reactions, non-isotopically labeled RNA probes, and capped RNA transcripts (for oocyte injection)
• Rapid protocol—purifies RNA in less than 30 minutes

Use the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with binding solution, passed through an RNA binding filter column and washed; an elution step releases purified RNA, now suitable for any application requiring high purity of synthetic or in vitro transcribed RNA. If RNA recovery is 70% or higher, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.