SUPERase•In RNase Inhibitor is a protein-based, non-human inhibitor that non-covalently binds to and inhibits common and The RNases that cause trouble include RNase A, B, C, 1, and T1.

SUPERase•In RNase Inhibitors can be used in any application where RNase contamination may be an issue. SUPERase•In inhibits RNase more broadly than traditional RNase inhibitors, making it the most effective RNase inhibitor available today and providing a higher level of protection against degradation.

SUPERase•In does not interfere with other enzymes such as RNA polymerase, reverse transcriptase or Taq DNA polymerase. In addition, SUPERase•In is active up to 65°C and in the pH range of 5.5 to 8.5.

If you plan to use this product with standard antibody purification methods, please contact Technical Support to discuss experimental design.

Unit Definition
Incubate in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA for 4 hours at 37°C, 1 U/μL The SUPERase•In RNase Inhibitor blocks the degradation of 0.1 μg/μL labeled RNA by 2.5 pg/μL RNase A, 2.5 pg/μL RNase I, and 0.0075 U/μL RNase T1. Analysis was performed by denaturing PAGE. SUPERase•In is currently the only ribonuclease inhibitor whose unit activity has been clearly defined by such functional assays.