Thermo Scientific 10X TBE Buffer (Tris-Borate-EDTA) is the ideal solution for DNA polyacrylamide gel electrophoresis and RNA polyacrylamide gel electrophoresis. A widely used buffer in acrylamide gel electrophoresis. TBE is used with either native gels or denaturing (7 M urea) gels. It is also routinely used in automated DNA sequencing gels. TBE can also be used in agarose gels, but is not recommended for use in preparative gels for recovering nucleic acids. Because the borate in TBE buffer is a strong inhibitor of many enzymes, when observing the application of enzymes in DNA samples, it is recommended to use TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647 ).

Applications

• Nucleic acid agarose gel electrophoresis and nucleic acid polyacrylamide gel electrophoresis
• Used as gel running buffer and gel preparation buffer Liquid
• Filter through 0.22 μm membrane
• Recommended for electrophoresis of RNA and DNA fragments less than 1500 bp

Note
Double-stranded linear nucleic acids Molecules move about 10% slower in TBE buffer than in TAE buffer.