Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosidic bond between uracil and sugar, This leaves an apyrimidine site in uracil-containing single- or double-stranded DNA (SeeFigure 1 in the Supporting Data). The enzyme is inactive against RNA

Product Benefits

• In Thermo Scientific buffers for restriction enzymes and thermophilic polymerases Active

Applications

• Control of residual contamination in PCR
• Glycosylase-mediated single nucleotide polymorphisms sex detection (GMPD)
• Site-directed mutagenesis
• Used as a probe for protein-DNA interaction studies
• SNP genotyping
• Cloning PCR products
• Generating PCR products and cDNA Single-stranded overhangs

Remarks

Abasic sites generated in DNA by Uracil-DNA Glycosylase may subsequently be heated, treated with alkali or in the nucleic acid Dicer (specifically cleaves at abasic sites). UDG (UNG) is active in the presence or absence of divalent cations.

Use of this enzyme in certain applications is protected by patent and may require licensing.