Thermo Scientific dsDNase is an engineered shrimp DNase designed to quickly and safely remove contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA, producing oligonucleotides with 5'-phosphate and 3'-hydroxy termini. High specific activity against double-stranded DNA, ensuring that RNA and single-stranded DNA (such as cDNA and primers) are not cleaved. dsDNase can be easily inactivated by moderate heat treatment (55°C). These characteristics make dsDNase a better choice for eliminating gDNA before reverse transcription. It greatly simplifies the workflow, optimizing the two steps of genomic DNA elimination and cDNA synthesis into a single-tube procedure.

Product Advantages

• Highly specific degradation of double-stranded DNA
• Rapid removal of gDNA 2 minutes before reverse transcription
• Thermolabile— Irreversible inactivation by moderate heat treatment (55°C)

Applications

• In first-strand cDNA synthesis, RT-PCR and RT-qPCR Removal of Genomic DNA from RNA Samples

dsDNase Specificity—The activity of dsDNase against double-stranded and single-stranded DNA and RNA oligonucleotides was measured. Relative activity values indicate that dsDNase is highly specific for double-stranded DNA.

• dsDNA - 100% relative activity
• ssDNA - <0.03% relative activity
• dsRNA - <0.01% relative activity
• ssRNA - <0.01% relative activity< /div>