Thermo Scientific terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes deoxyribose sugar Nucleotide repeats are added to the 3'-OH of oligodeoxyribonucleotides and single- and double-stranded DNA. TdT requires an oligonucleotide consisting of at least three nucleotides as a primer. When using RNA as a template, TdT shows different properties, mainly depending on the tertiary structure of the 3'-end of the acceptor RNA and the nature of the nucleotide. Typically, performance is lower than when using DNA as a template.

Product Advantages

• Incorporation of modified nucleotides (e.g., fluorescein, biotin, aminoallyl Labeled nucleotides)

Applications

• Production of synthetic homopolymers and heteropolymers
• Linear polymers with any type of 3'-OH terminus Homopolymer tailing of double helix DNA
• Oligodeoxynucleotides and DNA labeling
• 5'-RACE (Rapid Amplification of cDNA Ends)
• ApoptosisIn Situ< /i>Position

Remarks

TdT reaction buffer is not compatible with downstream applications due to the presence of CoCl₂. CoCl₂ from the reaction mixture must be removed by spin column or phenol/chloroform extraction and subsequent ethanol precipitation.