5'			G	A	C	G	C	N5	↓		3'
3'			C	T	G	C	G	N10	↑		5'

Thermo Scientific CseI (HgaI) restriction enzyme recognizes GACGC(5/10)^ site, in R buffer at 37°C The cleavage effect in liquid is better (isocleavage enzyme: HgaI). See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: Also available as FastDigest enzyme for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: Detection uses pBR322 DNA (SD0041). Star activity may occur at low salt, high concentrations of glycerol (>5%), pH >8.0, or excess enzyme. CseI may bind to severed DNA. This can cause DNA band drift during electrophoresis. To avoid atypical DNA banding patterns, please use 6X DNA loading dye and SDS solution to prepare samples, or add SDS to the DNA digestion product and heat it before electrophoresis. For methylation sensitivity, see product specifications.