Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high-throughput PCR applications . It outperforms all Taq -based hot-start polymerases on the market. Phire Hot Start II DNA Polymerase delivers powerful performance and speed to successfully amplify long DNA fragments with high yields. These features are achieved through advanced protein engineering of polymerases. It incorporates a unique double-stranded DNA binding domain that is compatible with Taq DNA polymerase Compared with this method, the extension time can be shortened (10 to 15 s/kb), the yield can be improved, and the fidelity can be increased by 2 times.

Phire Hot Start II Hot start modification of DNA polymerase is based on Affibody inactivation technology. This technology improves the specificity of PCR reactions without requiring additional initial enzyme activation time.

Product Advantages

• Fast Hot Start—no reactivation step required
• Quick Enzyme—Four times faster than hot-startTaq enzyme amplification
• Robust—Minimizes reaction optimization due to high inhibitor tolerance
• High Yields—The high efficiency of the enzyme results in large amounts of product
• Longer PCR product fragments—The length of the amplified DNA fragments is much longer than any hot startTaq

Applications

• Hot start PCR
• Conventional PCR
• Non-high fidelity PCR
• Fast PCR
• High-throughput PCR
• Genotyping

Note: The optimal annealing temperature ofPhire DNA polymerase may differ from that of Taq Polymerases are significantly different. For best results, first use our Tm CalculatorAccurately calculate your Tm.

Phire Green Hot Start II DNA Polymerase (F-124S or F-124L) is also available for loading PCR products directly onto the gel.