Thermo Scientific Phusion High-Fidelity DNA Polymerase sets the "gold standard" for efficient PCR. Phusion High-Fidelity DNA Polymerase has an error rate that is 1/50 that of Taq and 1/6 that of Pfu, making it ideal for cloning and other applications requiring high fidelity. Best choice. Compared to other DNA polymerases, Phusion DNA Polymerase has stable performance, less time-consuming protocols (even in the presence of PCR inhibitors), and can achieve higher yields with lower enzyme amounts.

Phusion High-Fidelity PCR Master Mix is a convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer containing MgCl₂. Two master mix formulations are available - HF-containing buffer (F-531S and F-531L) and GC-containing buffer (F-532S and F-532L). The mismatch rate of Phusion DNA polymerase in HF buffer (4.4 × 10^-7) was lower than that in GC buffer (9.5 × 10^-7) . Therefore, a master mix containing HF buffer should be used as the default master mix for high-fidelity amplification. However, GC buffer can improve the amplification performance of Phusion DNA polymerase on some complex templates or long templates, such as GC-rich templates or templates with complex secondary structures.

Product Advantages

•High fidelity (50X Taq)
•Short extension time (15-30 s/kb ), enabling fast PCR
• Stable performance, almost no need for optimization

Application

• High-fidelity PCR
• Complex amplification (high GC content) template
• Cloning
• Generate sequencing template
• Long fragment PCR (up to 20 kb)
• Mutation
• High-throughput PCR

Using Phusion DNA Polymerase
The annealing rules of Phusion DNA Polymerase are different from those of many common DNA polymerases, such as Taq DNA Polymerase. For best results, use www.thermofisher.cn/tmcalculator.