Medium 199 was originally developed for nutritional research on chicken embryo fibroblasts. It has broad species applicability and is particularly suitable for the culture of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro culture of primary explants of mouse pancreatic epithelium and rat lens tissue. We offer a variety of Gibco™ 199 modified media for a wide range of cell culture applications. Use Base Selection ToolFind a suitable recipe.
Full available Formulation.

Compared with other basal media, 199 medium contains unique components, including adenine, adenosine, hypoxanthine, thymine and other vitamins. This 199 medium formulation contains Hanks Balanced Salts for use in CO₂-free culture environments.

cGMP Manufacturing and Quality System
Gibco™ 199 Medium is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer the same Gibco™ 199 media product (22350-029) manufactured in our Scottish facility. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

199 medium does not contain proteins, lipids or growth factors. Therefore, 199 culture medium requires additives, usually 10% Fetal Bovine Serum (FBS). This 199 medium formulation does not require a CO₂ environment to maintain physiological pH.