Minimum Essential Medium (MEM) α is widely used in mammalian cell culture and selection of transfected DHFR-negative cells. MEM α can be used on a variety of suspended and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM alpha modified media suitable for a wide range of cell culture applications. Use
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The improvement of this MEM α is as follows:
Includes | Does not contain |
• Ribonucleosides | • Phenol red |
• Deoxyribonucleosides | |
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Full
formulation.
Gibco™ MEM alpha is a modified version of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B
12, biotin and ascorbic acid . MEM α is also available in a nucleoside-free version for use as a selection medium for DG44 and other DHFR-negative cells. This product is made from Earle's Balanced Salt.
Product UsageResearch use only: not for diagnostic or therapeutic use in humans or animals.
cGMP Manufacturing and Quality SystemsGibco™ MEM α is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.
MEM alpha medium does not contain proteins, lipids or growth factors. Therefore, MEM α requires supplementation, usually 10%
Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.