FIX & PERM™ Cell Permeabilization Kit is used for flow cytometric analysis to fix and permeabilize cells in suspension cells, allowing antibodies to enter intracellular targets without affecting scattering properties.

FIX & PERM™ Cell Permeabilization Kit:

• Flexible—compatible with a variety of sample types and cell antigens
• Robust—cell scattering patterns are not affected
• Efficient—can simultaneously detect intracellular and surface markers

ViewSelection guide for all available FIX & PERM™ products and related Products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information on cell isolation using Dynabeads™ cell isolation products).

FIX & PERM™ formulations are specifically designed to reduce background staining by adding fluorochrome-conjugated antibodies simultaneously during the permeabilization step. FIX & PERM™ Cell Permeabilization Kit is suitable for a variety of biological samples and is validated for use with cell lines, whole blood, and bone marrow. FIX & PERM™ Kits have been shown to accurately identify previously undetectable intracellular markers. Unlike other fixation and permeabilization reagents available on the market, FIX & PERM™ staining protocols leave the morphological scattering properties of cells intact.

How it works
FIX & PERM™ Cell Permeabilization Kit contains 2 reagents: fixed medium (Medium A) and permeabilization medium (Medium B ). Cells were incubated with fixation medium for 15 min at room temperature, washed, and then incubated with permeabilization medium and fluorochrome-labeled antibodies. Fixation medium and permeabilization medium are also available separately (Cat. No. GAS001S100 and GAS002S100).