Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kits are designed for entry-level cloning with Gateway™ Perform rapid cloning followed by high-level prokaryotic expression controlled by the strong phage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET300/NT-DEST and pET301/CT-DEST, respectively) to facilitate purification and detection (Figure 1). This vector kit is ideal for structural biologists who wish to make only minimal or no modifications to their proteins of interest. To maximize expression, use with MagicMedia™ E. coli Expression Medium.



Contents and Storage:
Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit includes pET300/NT- 6 µg each of DEST and pET301/CT-DEST vectors and 10 µg control vector. Store at -20"C. Guaranteed storage stability for 6 months when properly stored.