Using PicoGreen dsDNA Quantification Kits and Reagents, dsDNA as low as 0.25 pg/uL can be rapidly detected, even in the presence of ssDNA, RNA, and free nucleotides. This quantitation kit is linear over three orders of magnitude and has virtually no sequence dependence, allowing you to accurately measure DNA from a variety of sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.
PicoGreen dsDNA Quantitative Reagent has the following characteristics:
• Sensitivity is significantly higher than UV absorbance readings, saving precious sample usage
• Detection of dsDNA in the presence of RNA Specific
• Easy to use—simply add dye working solution to sample, incubate for 5 minutes, and read
• Available in 96- and 384-well plate formats
• Compatible with most fluorophores Microplate Readers and Fluorometers
• When combined with dsDNA, Picogreen has an approximate fluorescence absorption/emission maximum of 502/523 nm
Applications
PicoGreen dsDNA Quantification Reagents and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantification, dsDNA determination in complex mixtures, and viral DNA quantification.
For Research Use Only. Not for use in diagnostic procedures.