Labeled calcium indicators are molecules that show an increase in fluorescence upon binding to Ca²⁺. They can be used for a variety of calcium signaling studies, including determination of Ca²⁺ in cells and tissues with high levels of autofluorescence and Ca²⁺ produced by photoreceptors and photoactivated chelators. sup> release detection. These can be transferred using physical methods such as patch pipettes, microinjection, or Influx™ Cell Pinocytosis Loading Reagent A non-cell permeable salt form of the indicator is loaded into the cells. The fluorescent signal of these cells is usually detected by fluorescence microscopy.

Learn more about Ionic Indicators for Calcium, Potassium, pH and Membrane Potential Indicators›

Calcium Indicator (Cell-Impermeable Salt) Specifications:
• Label (excitation/emission wavelength of Ca²⁺–bound form): Rhod-5N (551/576 nm)
• Fluorescence after binding to Ca²⁺ Intensity increase: >100-fold
• K_d of Ca²⁺ without Mg²⁺ in buffer: ∼320 µM
• After binding Ca²⁺, the fluorescence increases and the wavelength changes slightly

Use TPEN to control heavy metal cations
In addition , BAPTA-based indicators can bind various heavy metal cations (such as Mn²⁺, Zn²⁺, Pb²⁺) with far-reaching affinity. Higher than Ca²⁺. The heavy metal selective chelator TPEN can be used to control perturbations in calcium measurements caused by these ions.

More choices of fluorescent calcium indicators
We provide a large number of Molecular Probes™ calcium indicators for use in various experimental scenarios. For more information, see Visible light excited fluorescent Ca²⁺ indicators—Chapter 19.3 section.

For UV excited Ca²⁺ indicators, protein-based Ca²⁺ indicators, Ca²⁺ indicators Conjugates of reagents and fluorescent indicators of other metal ions (i.e. Mg²⁺, Zn²⁺), please see Ca²⁺, Mg²⁺, Zn²⁺, and other metal ion indicators—Chapter 19.

For scientific research use only. Not for therapeutic or diagnostic use in humans or animals.