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Super Bright Complete Staining Buffer eBioscience is designed for use as a flow cytometry staining buffer in immunofluorescent staining protocols for suspension cells. of supplement. The use of Super Bright Complete Staining Buffer is only required when using multiple polymer dye-conjugated antibodies in the same sample to prevent non-specific polymer interactions, which may result in undercompensation of the data. Super Bright Complete Staining Buffer is available in convenient 5 µL/assay format and is compatible with traditional fluorescent dyes, Brilliant Violet dyes, and standard flow cytometry protocols.
Super Bright Complete Staining Buffer is not compatible with UltraComp eBeads Beads. If UltraComp eBeads beads are used as a compensation tool, all dilutions and washes should be made using only flow cytometry staining buffer during bead staining.
Super Bright Complete Staining Buffer is a fully compatible replacement for Super Bright Staining Buffer (Cat. No. SB-4400-42, SB-4400-75) and displays background in the 450/40 channel of violet lasers reduce.
Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.