Thermo Fisher 11430030 500 mL MEM (10X), Glutamine-free
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11430030 500 mLMEM (10X), Glutamine Free

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Minimum Essential Medium (MEM) is a commonly used cell culture medium. MEM can be used to culture a variety of suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes wait. Gibco™ MEM Modified Medium is available in a variety of components for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.

The improved form of this MEM is as follows:

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IncludesDoes not contain
• Phenol Red • L-Glutamine
• HEPES: < /td>
• Sodium bicarbonate
< br>Provide complete formulation.

Gibco™ MEM was developed by Harry Eagle based on his earlier Basic Eagle Medium (BME) formulation. Many other modifications of MEM have since been made, including Glasgow's MEM, MEM alpha, DMEM, and Temin's modified medium. MEM contains Earle's Balanced Salts for CO2 incubators, or Hanks' Balanced Salts for CO2-free incubators. This product is made with Earle's salt.

Use MEM
MEM does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Concentrated forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration when prepared (see Protocol).

cGMP Manufacturing and Quality Systems
Gibco™ MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Gibco™ MEM product (21430-020) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.