pcDNA™ vectors enable high-level constitutive expression in a variety of mammalian cell lines. Gateway™ pcDNA™-DEST40 vectors have the following key features:

•C-terminal V5-6x His tag facilitates detection and rapid purification
•Cytomegalovirus (CMV) promoter enables high-level expression
br>•TheattR site is used for Gateway™ cloning, allowing recombination using the attL-side region fragment
•Neomycin resistance gene for stability Screening
•Ampicillin resistance gene and pUC replication region sequences for E. coli screening and maintenance

Gateway™ Cloning
For all your expression needs, Invitrogen offers advanced Gateway™ purposes Vectors for expression in E. coli, insect, yeast or mammalian cells and for the generation of native proteins or N- or C-terminal fusion proteins. Whether entry or Ultimate™ ORF clones, all Gateway™ destination vectors have anattR site for recombination using anyattL-side fragment. The table below lists a range of available destination vectors.

Additional materials required, available for purchase separately:Gateway™ Starter Clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.