Select if you want to covalently couple antibodies, peptides, intact proteins, and functional enzymes to the bead surface Dynabeads™ Tosylactivated. Optimized antibody orientation makes these beads ideal for immunoprecipitation of proteins and protein complexes.

Ideal for immunoprecipitation of protein complexes:
Low background and covalent attachment of antibodies to the bead surface make Dynabeads Tosylactivated ideal for immunoprecipitation of proteins and protein complexes ( Excellent choice for co-immunoprecipitation, co-IP). Gentle and rapid magnetic aggregation of beads and extremely short incubation (possibly due to fast surface binding kinetics) make Dynabeads Tosylactivated excellent for immunoprecipitation of extremely unstable and/or transient (ephemeral) protein complexes. select.

Ultra-fast protocols:

• Time to identify protein complex members in minutes rather than hours
• Separation times are short enough to identify transient and intermittently Stable complexes
• Identification of binding partners not identified by long-term protocols
• Fast protocols further reduce already very low background binding
• Greater signal-to-noise ratio

Applications :
• IP of proteins and protein complexes
• Coupling functional enzymes to the bead surface for downstream analysis
• Coupling peptides to the bead surface to identify binders< br>
Purify peptides, proteins and enzymes that are:
• Transiently stable
• Structurally intact
• Poor temperature stability
• Have native configuration And fully functional

Overview of the coupling process:
Covalent coupling is achieved by overnight incubation of the selected ligand and Dynabeads Tosylactivated. Ligands coupled to Dynabeads Tosylactivated typically include peptides and proteins (such as antibodies used for immunoprecipitation or co-immunoprecipitation). Coupling occurs at neutral to high pH and 37°C. We recommend coupling at pH 8.5-9.5, but for ligands with poor pH stability, coupling can be performed in other buffers at pH 7.4.

After the ligand coupling step is completed, the actual surface coating of Dynabeads Tosylactivated is no longer active, so non-specific binding is very low.

About Dynabeads:
Dynabeads are nonporous monodisperse superparamagnetic beads. They are highly mobile in solution, allowing ligands coupled to the beads to continuously interact with the entire sample. By transferring the test tube to a rack containing a strong magnetic field (navigate Brands > Dynal;™> Magnetic Rack from the red navigation bar), the superparamagnetic beads are adsorbed to the tube wall. The strong magnetic field quickly adsorbs the beads to the tube wall, so all the supernatant can be easily removed by pipetting. The washing steps are similar.

Dynabeads Tosylactivated microbead surface properties:
•Tosyl (Tosyl) group
•Hydrophobic, pH neutral
•Via primary amine (NH2) or sulfhydryl (SH) groups covalently bound

Binding capacity per mg of beads:
Varies with ligand (e.g. 5-10 µg IgG )