Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of human in vitro expression systems and expressed recombinant c-Myc tagged proteins in bacterial and mammalian cell lysates.

c-Myc Sepharose Features:

•Specificity—Highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yields and High-purity immunoprecipitation
• Scalable—Available in 2 and 10 mL resin packs for larger-scale purification or immunoprecipitation
• General Purpose —Can be used with spin or gravity columns as well as FPLC purification columns
• Convenience —Reagents for elution and detection of c-Myc tag fusion proteins are available separately

for production Pierce anti-c-Myc Sepharose anti-c-Myc antibody is a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes c-Myc derived from the human c-Myc oncogene (p62 c-myc) Epitope tag (EQKLISEEDL). The carrier is Superflow 6 resin, a highly cross-linked 6% agarose resin. This anti-c-Myc affinity resin can be packed into gravity purification columns, centrifugal purification columns or purification columns of FPLC instruments to purify c-Myc fusion proteins expressed in bacterial or mammalian cells.

Application:
• Pierce Human In Vitro Translation Kit Purification of c-Myc fusion proteins
• Large-scale purification of recombinant c-Myc tagged proteins
• High-throughput enrichment of fusion proteins and interaction partners
• IP and co-IP experiments (with Pierce c-Myc Tag IP/co-IP KitSame)

Pierce Anti- c-Myc Sepharose consists of a highly specific monoclonal anti-c-Myc antibody covalently immobilized on a cross-linked 6% beaded agarose support. Product is available as a 25% slurry. After incubation of the sample, the c-Myc-tagged fusion protein is captured on agarose beads. After a simple wash step, tagged proteins are easily eluted from the resin using 0.1M Glycine (pH 2.0-2.8), 3M NaSCN, or 50 mM NaOH, depending on the downstream application of the purified protein. For elution of highly functional proteins, Pierce c-Myc Peptide can also be used to tag c-Myc of proteins are eluted. Anti-c-Myc antibodies can be used to detect the presence of tagged proteins by Western blot analysis.

In experiments using c-Myc-tagged fusion proteins (26 to 29 kDa), the resin provided a binding capacity of 102 to 144 nmol protein/mL of precipitated resin. Using 0.1M glycine pH 2.8, the elution capacity is at least 18 to 19 nmol per mL of precipitated resin. Binding and elution capabilities will vary depending on the c-Myc fusion protein and elution method.

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Applicable to Pierce™ anti-c-Myc Agarose c-Myc peptide control