Thermo Fisher Scientific Pierce GST-Tagged Protein Interaction Sedimentation Kit contains a method for capturing and purifying proteins that interact with GST-tagged fusion proteins. required components.

GST-Tagged Protein Interaction Sedimentation Kit Features:

•GST Sedimentation (Product No. 21516)—Gentle purification of any GST-tagged fusion protein (not denatured) Protein interactors
•Complete kit—Provides all components and detailed protocols for purified proteins: protein interactions
•No special equipment required— Use common laboratory equipment and reagents (e.g., microcentrifuge)
•Convenience—High-volume spin columns facilitate simple and efficient manipulation of agarose beads, including simple multi-sample processing
•Flexible—Instructions include protocols for use with bait and prey proteins from many different sources
•Conjugation—Conjugate approximately 8 mg of GST tag fusion per ml of resin Protein

Applications:
• Discovery of a new protein from cell lysates: protein-protein interacting proteins
• Confirmation of protein from cell lysates or previously purified proteins Putative Interactions
• Protein Extraction from In Vitro Transcription/Translation Lysates: Protein Interaction Information

You provide tagged fusion proteins as "baits" and express putative protein interactions To target ("prey") cells, the sedimentation kit provides, among other things: cell lysis buffer, microspin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocols. The sedimentation kit is designed to teach the method to first-time users and improve ease of use, convenience, and reproducibility for experienced researchers.

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