Thermo Scientific Pierce Anti-HA Agarose is an immunopurification and immunoprecipitation resin used to enrich Human in vitro protein expression system, HA-tagged proteins expressed in bacteria, yeast and mammalian cells. The resin-conjugated anti-HA antibody is a high-affinity mouse IgG1 monoclonal antibody that recognizes the HA epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.

Pierce Anti-HA Agarose Features
Specificity—Highly specific anti-HA monoclonal antibodies enable high yields and purity
Scalable —Available in 1 mL and 5 mL precipitation resin packages for smaller-scale immunoprecipitation or larger-scale purification
Universal—Can be used for centrifugation- or gravity-based purification and Enrichment Methods

Pierce Anti-HA Sepharose consists of a highly specific monoclonal anti-HA antibody covalently immobilized on a cross-linked 4% beaded agarose support. This product is supplied as a 50% slurry in 0.1 M phosphate, 0.15 M NaCl (pH 7.2) containing 0.05% sodium azide. Anti-HA Sepharose Precipitates 3.5 mg of mouse anti-HA IgG1 monoclonal antibody per mL of agarose resin.

HA-tagged proteins can be easily eluted from the resin using 0.1 M Glycine (pH 2.0–2.8), 3 M NaSCN, or 50 mM NaOH, depending on the downstream application of the purified protein. For mild elution, HA synthetic peptide can be used for neutralization and from immobilized anti-HA affinity Competitive elution of HA-tagged fusion proteins from the resin.

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