Thermo Scientific EZ-Link Amine-PEG2-Biotin is a water-soluble biotin compound containing polyethylene glycol ( PEG) spacer arm and terminal primary amine, coupled via EDC and other cross-linker methods.

EZ-Link Amine-PEG2-Biotin Features:

•Biotinylation—for use in probes involving avidin or streptavidin Labeled molecules and surfaces for needle and resin detection or affinity purification methods
•Amine Activation—Primary amines can be cross-linked to proteins and material surfaces using EDC and other cross-linking agents
• PEGylated—Polyethylene glycol (PEG) groups in the spacer arm increase the water solubility of the biotinylated molecule
• Medium length—Spacer arm (added to the target) is 20.4 Å

Amine-PEG2-biotin and amine-PEG3-biotin are three compounds containing Polyethylene glycol (PEG)The shorter of the two amine-modified biotin compounds with spacer arms. Short PEG fragments are hydrophilic, conferring higher solubility on the labeled protein compared to reagents containing only hydrocarbon spacers. Use EDC (Product No. 22980), a water-soluble carbodiimide cross-linker. The primary amine of these pegylated biotin reagents is coupled to the carboxyl terminus, aspartate residue, or glutamic acid residue. EDC activates the carboxyl group to combine with the –NH2 group of amino-biotin to form an amide bond.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

Amino-biotin compounds can be coupled to functional groups on proteins and other molecules in a variety of ways. The more common method is to use Cross-links terminal primary amines to carboxyl groups. Carboxyl groups (-COOH) are present on aspartic acid or glutamic acid residues and on the carboxyl termini of polypeptides. When activated withEDC (Product No. 22980), the carboxylate reacts with the amino group (— NH2) group reacts to form an amide bond. Carboxylate molecules and surface materials can be prepared using EDC and Sulfo-NHS (Product No. 24510) Activated for subsequent reactions with primary amines (see NHS Ester Chemistry).

Related products
EZ-Link™ Amine-PEG3- Biotin