Thermo Scientific SuperSignal West Pico PLUS Chemiluminescence Substrate is an enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate that detects picogram to high femtogram levels of protein by Western blot analysis.

SuperSignal West Pico PLUS chemiluminescent substrate features include:
•Sensitivity: Picogram to femtogram level sensitivity
• Stability: b>Working solution remains stable for 8 hours at room temperature; kit remains stable for 1 year
•Compatibility:Nitrocellulose and PVDF membrane
•Signal duration :6-24 hours
• Recommended primary antibody concentration:1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration:1:20,000–100,000 dilution (10–50 ng/mL)

Compare and learn more about Western blotting chemiluminescent substrates›
View all available HRP substrates›

SuperSignal West Pico PLUS substrates are designed to provide excellent signal strength and sensitivity for use in HRP conjugates were subjected to Western blot analysis. The combination of luminous intensity and excellent duration enables multiple exposures, making it easier to obtain publication-quality blot images. SuperSignal West Pico PLUS Substrate is compatible with different membranes, blocking reagents and various antibody dilutions, making it ideal for most Western blotting applications.