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How SMART Digest Pepsin Kit Works
Pepsin preferentially cleaves proteins at the carboxyl group of aromatic amino acids such as phenylalanine, tryptophan, and tyrosine. It does not cleave at bonds containing valine, alanine, or glycine. Pepsin is commonly used to generate antibody fragments. Pepsin generates F(ab')2 fragments by removing only the portion of the Fc domain beyond the hinge region. The resulting F(ab')2 fragment consists of two antibody-binding Fab' fragments linked to disulfides in the hinge region.