The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. PichiaPink™ Secretion Signal Sets are designed to be easily attached to the front of genes. The PichiaPink™ Secretion Signal Set is designed for use with pPINK-HC and pPINK-LC vectors.

Optimize secretion of your expressed protein
By adding one of 8 secretion signal sequences to your gene, you can secrete your protein into the culture medium , to facilitate harvesting and purification. Pichia pastoris secretes very few natural proteins, so most of the proteins in the culture medium are the ones you need.

Each DNA sequence encodes a different secretion signal. You can examine each secretion signal sequence to find the one that is more effective for your protein.

PichiaPink™ Secretion Signal Set features 8 different secretion signals including:
• Saccharomyces cerevisiaeα-mating factor pre-sequence
• Aspergillus nigerα-amylase signal sequence
• Aspergillus awamoriGlucoamylase signal sequence
• Homo sapiensSerum albumin signal sequence
• < i>Kluyveromyces marxianus Inulinase signal sequence
• Saccharomyces cerevisiaeInvertase signal sequence
• Saccharomyces cerevisiae Killer protein signal sequence
• Jungle FowlLysozyme Signal Sequence

Easily Add Secretion Signal to Your Protein
PichiaPink™ Secretion Signal Set DNA Fragments Designed to easily insert in front of your gene when using pPINK-HC or pPINK-LC vectors. You can try different secretion signal sequences to determine which one results in better secretion of your protein.

Each signal sequence includes the Kozak sequence from the AOX1 gene of Pichia pastoris to ensure efficient translation of the protein.

The secretion signal sequence is presented as a phosphorylated double-stranded oligomer in a 40 pmol aliquot. Resuspend oligomers in 40 µl TE buffer before use.

Why choose PichiaPink™ yeast expression system?
PichiaPink™ yeast expression system is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and ease of handling. For more than 30 years, Pichia pastoris has been used by laboratories around the world to produce hundreds of different proteins from multiple species, including humans (Refs. 1, 2). The PichiaPink™ yeast expression system enables you to produce proteins from small to large scale in a convenient and cost-effective manner.

For information on obtaining a commercial license for the PichiaPink™ Yeast Expression System, please visit outlicensing@lifetech.com.

Research use only. Not intended for use in the treatment or diagnosis of any animal or human.

Related links
•See Learn more about PichiaPink™ Yeast Expression Systems.
• Learn more about other protein expression systems.

References

1.Cereghino JL, Cregg JM.Heterologous protein expression in the methylotrophic yeast Pichia pastoris.FEMS Microbiol Rev. 2000 Jan;24(1) :45-66.[PubMed]
2.Cereghino GP, Cereghino JL, Ilgen C, Cregg JM.Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.Curr Opin Biotechnol.2002 Aug;13(4):329-32.[PubMed]