Alexa Fluor Plus 555 Phalloidin is a high-affinity F-actin probe Needle, coupled to our bright, photostable orange/TRITC fluorescent Alexa Fluor Plus 555 dye. In order to achieve ideal sensitivity and specificity, when synthesizing Alexa Fluor Plus 555 phalloidin, try to ensure that the number of Alexa Fluor Plus 555 phalloidin bound to F-actin per unit length is as high as possible.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidins for F-actin staining›

• Go beyond Alexa Fluor 555 phalloidin selectively stains F-actin with 3–4 times the sensitivity
• Excellent fluorescence performance, ensuring clear images of the actin cytoskeleton
• Excitation/emission wavelength: 555/565 nm. Compatible with standard TRITC/RFP filter sets or laser settings
• Increased sensitivity and specificity for actin relative to antibody staining
• Best for fixed and permeabilized samples

Excellent actin staining results
Phalloidin is a bicyclic peptide isolated from the deadly Amanita “death cap” mushroom Toxin, often used to selectively label F-actin in imaging applications. Compared with antibodies, fluorescently labeled phalloidin has several advantages in labeling actin, including: its binding properties to animal and plant actin from different species are almost the same, and the non-specific binding ratio is low. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides ultra-bright and photostable orange/TRITC fluorescence.

Can be used in a variety of applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantify F-actin activity in tissue sections, cell cultures, or dissociated preparations protein. The staining is fully compatible with other fluorescent stains used in cell analysis procedures, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates (including secondary antibodies).