Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal culture base that can be used to support the growth of many types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and mouse fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.

BenchStable DMEM/F-12 is designed to be stored at room temperature and support cell proliferation and maximum cell density as well as conventional DMEM/F12 supplemented with ≥10% fetal bovine serum (FBS).

This DMEM/F-12 is modified in the following ways:
• Optimized for room temperature stability
• Contains GlutaMAX, Phenol Red
• Does not contain HEPEPS

Usage of BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula combines DMEM's high concentration of glucose, amino acids and vitamins with a broad spectrum of F-12. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires an environment of 5–10% CO2 to maintain physiological pH. Contains GlutaMAX additive< /a>'s DMEM/F-12 is available in an easy-to-use package that significantly reduces toxic ammonia accumulation and improves cell viability and growth. BenchStable DMEM/F-12 does not contain protein, lipids or growth factors and therefore requires ≥10% supplementation Fetal bovine serum (FBS).

cGMP Manufacturing and Quality Systems
Gibco BenchStable media are manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

For more information about Gibco BenchStable media, please visit thermofisher.cn/benchstable.