The Ambion™ DNaseAlert™ QC System is a patent-pending technology that enables fluorescence measurement in an easy-to-use Sensitive detection of DNase and provides results in real time. Contains sufficient reagents for 5 × 96 high-throughput assays.

• Detect a range of different DNases in minutes
• Certify plastics, enzymes, solutions and other biological materials as “DNase-free” prior to DNase-sensitive applications such as PCR
• With RNaseAlert™ Combination for Simultaneous Quantitative Analysis of RNase and DNase Activities

The DNaseAlert™ QC System contains a unique substrate that is simultaneously labeled with a fluorescent reporter gene and a dark quencher. Due to the proximity of the fluor and quencher, the uncleaved substrate emits very little fluorescence. When DNase is present, the bond between the fluor and the quencher is severed, resulting in a bright orange signal when excited at the appropriate light wavelength. The extent of substrate cleavage is proportional to the level of DNase contamination and can be monitored by increased fluorescence on a fluorometer.

Quick and easy solution
DNaseAlert™ QC System solution is fast and simple. Substrate was reconstituted in TE buffer and aliquoted into 96-well plates containing reaction buffer. The test sample is then added and the fluorescence is monitored to determine the assay result. Sufficient substrate is provided for 500 reactions. The DNaseAlert™ QC System also includes negative and positive controls for accurate identification of contaminated samples. DNaseAlert™ is a kinetically reliable assay. Data can be collected in real time, and the initial speed is dose-dependent on the amount of input DNase I.

Accessory Products
DNaseAlert™ is designed to work seamlessly with the RNaseAlert™ QC System (SKU# AM1966) and allows simultaneous analysis of DNase and RNase in the same sample Two-color detection. The fluorescent reporter linked to DNaseAlert™ Substrate has a different spectrum than the fluorescent label on RNaseAlert™ Substrate.