Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.
This Ham's F-12 is modified as follows:
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10%
Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g / L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.
Gibco® 'BASIC' products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.
For Research Use Only. Not for use in diagnostic procedures.