EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent containing a dibenzylcyclooctyne ( DBCO) group for copper-free click chemistry to attach to azide-tagged molecules, enabling the attachment of biotin using a bioorthogonal approach. The DBCO group reacts preferentially with azide via the copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form stable triazole bonds with azide-labeled coupling partners. EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and range.

EZ-Link Biotin-PEG12-DBCO Features include:
• Bioorthogonal—DBCO and azide selectively react to form triazole bonds
• Enhanced solubility—PEGylation Making biotinylated molecules water-soluble helps prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms irreversible and stable triazole bonds

Classic click reactions include copper Catalytic azide-alkyne cycloaddition to label or couple molecules. The disadvantage of this method is that copper ions can impair protein function, damage cells, and reduce the fluorescence of fluorophores. DBCO is unique in that it does not require copper to catalyze the reaction with azide to form the triazole. The eight-membered ring form of the strain allows reaction with azide-modified molecules without a catalyst, thereby preventing copper-induced damage to cells or proteins.