< td>

5'		T ↓	C	G	A		3 '
3'			A	G	C ↑	T		5'

Thermo Scientific TaqI restriction enzyme recognizes the T^CGA site and cleaves at 65°C in its unique buffer optimal. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Restrictions Reaction conditions for sexual endonuclease. Note: FastDigest enzyme for rapid DNA digestion is also available.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Notes: Experiments were performed using lambda DNA (dam-) (#SD0021). TaqI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163. 10% active when incubated at 37°C. We recommend using TaqI, HC at 37°C. See product specifications for methylation sensitivity.