5'			G	A	A	G	A	N8	↓		3'
3'			C	T	T	C	T	N7	↑		5'

Thermo Scientific MboII restriction enzyme recognizes the GAAGA(8/7)^ site in B buffer at 37°C. Best cutting results. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Restrictions Reaction conditions for sexual endonuclease. Note: FastDigest enzyme is also available for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Product Benefits

• Superior quality—strict quality control and industry-leading manufacturing processes
• Convenient color-coded five-buffer system
• Included for dual Universal Tango Buffer for digestion
•Premix BSA in reaction buffer
•A variety of restriction endonuclease specificities available

Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism (RFLP)
•SNP

Remarks:
Experiments were performed using lambda DNA (dam-) (#SD0021). MboI is blocked by overlapping dam methylation. To avoid dam methylation, use dam-, dcm- strains such as GM2163. MboI, Bsp143I, and DpnI all recognize the same sequence but have different methylation sensitivities and cleavage sites. See product specifications for methylation sensitivity.