Thermo Scientific Phusion High-Fidelity DNA Polymerase sets the "gold standard" for efficient PCR. Phusion High-Fidelity DNA Polymerase has an error rate that is 1/50 that of Taq and 1/6 that of Pfu, making it ideal for cloning and other applications requiring high fidelity. Best choice. Compared to other DNA polymerases, Phusion DNA Polymerase has stable performance, less time-consuming protocols (even in the presence of PCR inhibitors), and can achieve higher yields with lower enzyme amounts.

Product Advantages

•Preparation of reaction system at room temperature
•No non-specific amplification and primer degradation during reaction system preparation
•With the help of Unique hot start technology, no need to wait for reactivation
•High fidelity (52X Taq)
•Short extension time (15-30 s/kb), enabling fast PCR
•Stable reaction, Little optimization required
•Higher product yields with minimal enzyme usage
•Available in green buffer format that can be loaded directly onto the gel (F-566S or F-566L)

Phusion Hot Start II high-fidelity DNA polymerase is a mixture of Phusion DNA polymerase and a reversibly binding specific Affibody ligand (which inhibits DNA polymerase activity at room temperature), so it will not amplify when using it Produce non-specific products. Affibody ligands also inhibit the 3´→5´ exonuclease activity of the polymerase, thereby preventing primer and template DNA degradation during reaction setup. At temperatures that promote polymerase activity, the ligand is released, allowing full activation of the polymerase. In addition, Phusion Hot Start II DNA Polymerase reactivates immediately at elevated temperatures, eliminating the need for a separate activation step in PCR protocols. Phusion Hot Start II High-Fidelity PCR Master Mix is a convenient 2X mix that minimizes the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides, and optimized reaction buffer (including MgCl₂). Just add template and primers to start the PCR reaction.

Applications

• High-fidelity PCR
• High-throughput
• Amplification of difficult (GC-rich) templates
• Generation Templates for sequencing
•Mulplex PCR
•Multi-range PCR
•Cloning
•Mutation
•Microarray

Using Phusion DNA Polymerase
The annealing temperature design rules of Phusion DNA polymerase are different from those of many ordinary DNA polymerases (such as Taq DNA polymerase). For best results, use www.thermofisher.cn/tmcalculator.